Table 2. Stability values and ranking of candidate reference genes were determined by BestKeeper, Normfinder and geNorm.
| miRNA | BestKeeper | geNorm |
NormFinder |
|||
|---|---|---|---|---|---|---|
| std dev [± CP] | Rank | Stability value (M) | Rank | Stability value | Rank | |
| hsa-miR-101-3p | 1.413 | 8 | 1.334 | 9 | 0.730 | 6 |
| hsa-miR-16-5p | 1.820 | 9 | 1.474 | 8 | 0.878 | 8 |
| hsa-miR-93-5p | 1.297 | 6 | 0.960 | 2 | 0.269 | 2 |
| hsa-miR-320d | 0.498 | 1 | 1.471 | 8 | 0.906 | 9 |
| hsa-miR-25-3p | 0.842 | 3 | 0.977 | 3 | 0.283 | 3 |
| hsa-miR-92b-3p | 0.748 | 2 | 1.327 | 6 | 0.757 | 7 |
| hsa-miR-106b-5p | 0.966 | 4 | 0.944 | 1 | 0.228 | 1 |
| hsa-miR-10b-5p | 1.267 | 5 | 1.135 | 5 | 0.498 | 5 |
| hsa-miR-107 | 1.394 | 7 | 1.113 | 4 | 0.420 | 4 |
The stability is indicated by standard deviation of crossing point (CP) values in Bestkeeper, a lower stability value (M) in geNorm and stability value in Normfinder.