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. 2016 Oct 19;6:35606. doi: 10.1038/srep35606

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Sample preparation steps of a rat lung lobe with the CRISTAL method. The accessory lung lobe of the rat is shown after fixation and after the resin-based clearing. (b) Workflow of the correlative study. First, the CRISTAL sample was screened by using SLOT. Based on the identified ROI, further investigations like MPM, histology or TEM were carried out. The CRISTAL block was adapted to the requirements of the various corresponding imaging techniques and sectioned for histology and immunofluorescence as well as TEM.