Figure 4. Administration of CNO leads to increased Ca²⁺-levels in podocytes in vivo.

(A) Glomeruli of ROSA26^hM3D/wt; NPHS2.Cre^tg/wt; GCaMP3^tg/wt mice were visualised in the intact kidney in vivo using a Multi-Photon-microscope. CNO (5 mg/kg) was injected i.a. after 10  sec and caused an immediate increase in intracellular Ca²⁺ levels (scale bar = 25 μm). (B) Fluorescence is depicted as F/F_1–10, where the mean value of the first ten measurements (F_1–10) prior to CNO stimulation was used for normalisation. Experiments were performed in three biological replicates. (C) Statistical analysis performed for Multi-Photon-imaging experiments revealed a significant increase of the intracellular Ca²⁺ after CNO stimulation. N = 18 cells were included in the statistical analysis (t-test p < 0.0001).