Figure 6. Increased intracellular Ca²⁺-levels do not cause glomerular disease.

(A) Scheme of CNO administration. 5 mg/kg bodyweight CNO was injected i.p. on 5 consecutive days. (B) Urinary albumin to creatinine ratios did not show an increase after 5 days of CNO administration in both groups. Displayed are means with SEM; 2way ANOVA and Bonferroni’s multiple comparison test (p > 0.05). (C) Kidney sections of hM₃D-expressing animals and their littermate controls after 5 days of CNO treatment were stained with a podocin and synaptopodin antibody to visualize the slit diaphragm and the actin cytoskeleton respectively (scale bar = 20 μm). (D) PAS (periodic acid Schiff stainings) were performed on day 5 of CNO administration and did not reveal differences in histology (scale bar = 50 μm).