Figure 7. Long-term CNO treatment does not cause glomerular disease.

(A) Scheme of CNO administration. 5 mg/kg bodyweight CNO was injected i.p. on 18 consecutive days. (B) Urinary albumin to creatinine ratios did not show an increase after 18 days of CNO administration in both groups. Displayed are means with standard error of the mean (SEM); 2 way ANOVA and Bonferroni’s multiple comparison test (p > 0.05). (C) Kidney sections of hM₃D-expressing animals and their littermate controls after 18 days of CNO stimulation were stained with a podocin and synaptopodin antibody to visualize the slit diaphragm and the actin cytoskeleton respectively (scale bar = 20 μm). (D) PAS (periodic-acid Schiff stainings) were performed on day 18 of CNO administration and did not reveal differences in histology (scale bar = 50 μm).