| Reagent | Volume |
|---|---|
| T4 DNA ligase buffer with 10nM ATP | 10 μL |
| T4 DNA Polymerase | 5 μL |
| T4 Polynucleotide Kinase (PNK) | 5 μL |
| dNTP mix (10mM each) | 4 μL |
| Klenow DNA Polymerase | 1 μL |
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