FIG 5 .
Relationship of the P-type ATPases from C. metallidurans. A multiple alignment of all P-type ATPases from C. metallidurans with all E. coli proteins, MgtA and MgtB from Salmonella, SERCA1a from Rattus norvegicus, and SLL1614 from the cyanobacterium Synechocystis was performed, showing the position of the three PIB2-type ATPases for export of lead (PbrA), zinc (ZntA), and cadmium (CadA); the PIB4-type zinc exporter CzcP; the anabolic (CtpA and RdxI) and detoxifying (CopF and CupA) copper exporter; the part of the potassium/sodium exchange system KdpB; and finally Rmet_2211 (MgtB) and Rmet_5396 (MgtA), which were much more closely related to SERCA1a than to MgtA and MgtB from Salmonella. The alignment was constructed with Geneious 6.1.6 (Biomatters Ltd., Auckland, New Zealand) using the Blosum 62 cost matrix with an open gap penalty of 12 and a gap extension penalty of 3, the Jukes-Cantor genetic distance model, and the neighbor-joining tree building method. Numbers of substitutions per site are indicated, along with a scale bar.