TABLE 4 .
Growth parameters of the mutant strainsa
| Bacterial strain | Growth rate (1/h)/duration of the lag phase (h) after addition of metal: |
|||
|---|---|---|---|---|
| None | 10 µM CoCl2 | 10 µM ZnCl2 | 2.5 µM CdCl2 | |
| AE104 | 0.29 ± 0.03/0 | 0.26 ± 0.07/0 | 0.23 ± 0.01/0 | 0.23 ± 0.06/0 |
| ΔzupT | 0.28 ± 0.06/1.0 | 0.26 ± 0.03/3.9 | 0.22 ± 0.04/0 | 0.22 ± 0.06/2.8 |
| Δ7 | 0.34 ± 0.15/6.1 | 0.21 ± 0.02/7.0 | 0.23 ± 0.01/0 | 0.20 ± 0.07/6.2 |
| Δ7 mgtA::kan | 0.26 ± 0.02/0.9 | 0.23 ± 0.06/0.5 | 0.24 ± 0.03/0 | 0.16 ± 0.04/0 |
| Δ8 | 0.29 ± 0.00/3.3 | 0.22 ± 0.03/1.0 | 0.21 ± 0.01/0 | 0.17 ± 0.04/0 |
| Δ9 | 0.23 ± 0.05/2.8 | 0.19 ± 0.06/10.9 | 0.27 ± 0.08/9.0 | 0.06 ± 0.01/0 |
a
Strain AE104 and its mutant derivatives were cultivated in TMM at 30°C without further additions or in the presence of added metal salts, and growth was measured as turbidity at 600 nm (Fig. 3). The mean data points of 3 reproductions were used to calculate the growth rate and duration of the lag phase.