TABLE 5 .
Resistance of deletion strains in liquid culturea
| Bacterial strain | IC50 (µM) of agent: |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Zinc | Cobalt | Cadmium | Copper | Goldb | Nickel | EDTA | H2O2 | Paraquat | |
| AE104 parent strain | 442 ± 40 | 94 ± 6 | 93 ± 10 | 906 ± 82 | 25.3 ± 2.1 | 348 ± 16 | 249 ± 9 | 2,650 ± 279 | 169 ± 10 |
| AE104 ΔzupT | 156 ± 16 | 34 ± 6 | 16 ± 3 | 400 ± 77 | 13.0 ± 1.3 | 171 ± 10 | 183 ± 13 | 894 ± 133 | 111 ± 6 |
| Δ7 | 53 ± 4 | 8.3 ± 1.6 | 2.19 ± 0.4 | 265 ± 32 | 14.8 ± 2.6 | 145 ± 16 | 205 ± 22 | 989 ± 102 | 135 ± 4 |
| Δ7 ΔmgtA::kan | 113 ± 8 | 43 ± 8 | 8.5 ± 0.8 | 449 ± 51 | 13.2 ± 2.4 | 352 ± 16 | 217 ± 32 | NDc | ND |
| Δ8 (=Δ7ΔmgtB) | 145 ± 9 | 45 ± 7 | 9.0 ± 0.7 | 444 ± 46 | 9.5 ± 2.4 | 367 ± 12 | 210 ± 11 | 421 ± 75 | 64 ± 5 |
| Δ9 (=Δ8ΔmgtA::kan) | 176 ± 27 | 3.7 ± 0.5 | 0.40 ± 0.04 | 681 ± 72 | 25.8 ± 5.2 | ND | 406 ± 43 | 491 ± 96 | 71 ± 10 |
a
Dose-response experiments were performed (n > 3 per condition), and the IC50 values were calculated. Boldface values indicate significant deviations from metal resistance of Δ7.
b
Au(III)Cl4−. The values for AE104, ΔzupT, and Δ7 strains have been published elsewhere (7) and are given for reference.
c
ND, not done.