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. 2004 Sep;24(17):7313–7323. doi: 10.1128/MCB.24.17.7313-7323.2004

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Copyright © 2004, American Society for Microbiology

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FIG. 8. — Effect of Id1 on thymocyte positive selection in AND transgenic mice. (A) Thymocytes from littermates with the indicated genotypes were stained with anti-CD4 and anti-CD8 antibodies. The numbers shown indicate the percentages of the subsets. The total thymic cellularity is shown in parentheses. Eight litters of mice were analyzed, and identical results were obtained each time. The data shown are representative of analyses for one such litter. (B) Detection of apoptosis. Total thymocytes from mice with the indicated genotypes were labeled with FITC-conjugated dUTP and analyzed by flow cytometry. The percentage of apoptotic cells is shown on top of the gate.