TABLE 1 .
PCR primer sequences used in this study (old and new constructs)
| Primer name | Primer sequencea | Reference |
|---|---|---|
| 515f Original | GTGCCAGCMGCCGCGGTAA | Caporaso et al. (1) |
| 806r Original | GGACTACHVGGGTWTCTAAT | Caporaso et al. (1) |
| 515f Modified | GTGYCAGCMGCCGCGGTAA | Parada et al. (4) |
| 806r Modified | GGACTACNVGGGTWTCTAAT | Apprill et al. (5) |
| 926r | CCGYCAATTYMTTTRAGTTT | Parada et al. (4) |
| ITS1f | CTTGGTCATTTAGAGGAAGTAA | Gardes and Bruns (6) |
| ITS2 | GCTGCGTTCTTCATCGATGC | White et al. (7) |
a
Primers are listed in a 5′-to-3′ orientation. The original 515f and 806r primers are listed for comparison to the added degeneracy in the new constructs (changes are shown in bold). See Smith and Peay (8) for further details on the modifications to the ITS sequencing primers.