FIG 6 .

Later isolates from the dominant clade, C3, exhibit higher c-di-GMP levels and less motility than earlier isolates. (A) Intracellular c-di-GMP concentrations in BM1 and clade C3 (BM11, BM12, BM20, and BM21) isolates following 5 h of growth, as measured by LC-MS/MS. Significantly greater c-di-GMP levels were detected in evolved isolates than in the first isolate, BM1 (*, P < 0.05; **, P < 0.01; ***, P < 0.001), by Tukey’s HSD multiple-comparison test. (B) Swimming motility (assayed in 0.3% of agar plates incubated at 37°C for 24 h) was significantly less for isolates from clades C2 to C4 than for isolates from C1 (which includes isolate BM1) (***, P < 0.001 by Tukey’s HSD multiple-comparison test).