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. 2004 Sep;24(17):7779–7794. doi: 10.1128/MCB.24.17.7779-7794.2004

FIG.9.

FIG.9.

Expression and function of HERP2 in erythroid differentiation of primary human CD34+ hematopoietic progenitor cells. (A) Expression of HERP2, GATA-1, and GATA-2 proteins in CD34+ cells cultured in erythroid medium for the indicated durations. Whole-cell lysates were analyzed by immunoblotting with the indicated antibodies. (B) HERP2-transduced CD34+ cells show impaired erythroid differentiation. Cells transduced with MSCV-HERP2-IRES-GFP were cultured in erythroid medium for 7 days, followed by flow cytometric analysis for the erythroid marker glycophorin A (GPA), as in Fig. 1. Results from parallel transduction with the control vector are shown in Fig. 1A. Similar results were obtained from two independent experiments. (C) c-Jun and HERP2 both interfere with erythroid maturation. CD34+ cells transduced with constructs encoding the vector, c-Jun, or HERP2 were cultured in erythroid medium for 7 days, followed by flow cytometric analysis with costaining for GPA plus CD13 (granulocytic lineage marker), GPA plus CD34 (immaturity marker), or GPA plus CD41 (megakaryocyte-associated antigen).