FIG. 5.

Reduced steady-state level of eRF1 following its expression from the CUP1 promoter as determined by Western blot analysis. Reduced expression of eRF1 was driven from the CUP1 promoter (PCUP1) in a sup45Δ strain, while the control strain expressed eRF1 from its natural promoter (PSUP45) at its genomic locus. Both strains were grown in SMD medium containing 50 μM EDTA to specifically reduce eRF1 expression from the CUP1 promoter. Twenty-five micrograms of total protein was loaded per lane.