TABLE 2.
Effect of eRF1 depletion on translation termination
| Stop signalb | % Readthrough fora:
|
Change (fold) | |
|---|---|---|---|
| PSUP45 | PCUP1 | ||
| UAAA | 0.19 ± 0.02 | 0.94 ± 0.05 | 5 |
| UAAC | 0.37 ± 0.06 | 2.8 ± 0.27 | 7.5 |
| UAAG | 0.22 ± 0.02 | 1.2 ± 0.07 | 5.3 |
| UAAU | 0.28 ± 0.01 | 0.97 ± 0.10 | 3.4 |
| UAGA | 0.18 ± 0.01 | 0.60 ± 0.05 | 3.3 |
| UAGC | 0.49 ± 0.02 | 2.0 ± 0.18 | 4.1 |
| UAGG | 0.13 ± 0.01 | 0.80 ± 0.04 | 6.5 |
| UAGU | 0.12 ± 0.02 | 0.70 ± 0.07 | 5.9 |
| UGAA | 0.37 ± 0.04 | 4.6 ± 0.50 | 12.5 |
| UGAC | 1.3 ± 0.12 | 11.7 ± 0.95 | 9 |
| UGAG | 0.55 ± 0.03 | 1.9 ± 0.23 | 3.5 |
| UGAU | 0.24 ± 0.05 | 1.2 ± 0.2 | 5 |
a
Percent readthrough is expressed as the mean ± standard deviation.
b
All measurements were carried out using the [psi−] strains YDB340 (wild type) or YDB447 (sup45Δ) carrying pDB811, which expresses eRF1 from the CUP1 promoter.