TABLE 3.
Effect of eRF3 depletion on translation termination
| Stop signalb | % Readthrough fora:
|
Change (fold) | |
|---|---|---|---|
| PSUP35 | PCUP1 | ||
| UAAA | 0.16 ± 0.02 | 0.78 ± 0.02 | 5.0 |
| UAAC | 0.15 ± 0.01 | 1.2 ± 0.1 | 8.2 |
| UAAG | 0.13 ± 0.02 | 0.71 ± 0.12 | 5.6 |
| UAAU | 0.08 ± 0.01 | 0.79 ± 0.07 | 9.6 |
| UAGA | 0.10 ± 0.02 | 0.64 ± 0.1 | 6.4 |
| UAGC | 0.20 ± 0.03 | 1.7 ± 0.17 | 8.3 |
| UAGG | 0.13 ± 0.03 | 1.0 ± 0.15 | 8.1 |
| UAGU | 0.06 ± 0.01 | 0.30 ± 0.03 | 5.4 |
| UGAA | 0.17 ± 0.01 | 1.3 ± 0.14 | 7.7 |
| UGAC | 0.35 ± 0.01 | 5.3 ± 0.5 | 15.1 |
| UGAG | 0.11 ± 0.02 | 1.8 ± 0.06 | 16.4 |
| UGAU | 0.07 ± 0.02 | 0.96 ± 0.2 | 13.3 |
a
Percent readthrough is expressed as the mean ± standard deviation.
b
All measurements were carried out using the [psi−] strains 614 (wild type) or YDB498 (sup35Δ) carrying pDB824, which expresses eRF3 from the CUP1 promoter.