TABLE 4.
Effect of eRF1 overexpression on translation termination
| Stop signalb | % Readthrough fora:
|
Change (fold) | |
|---|---|---|---|
| Wild type | High-copy-no. eRF1 | ||
| UAAA | 0.18 ± 0.03 | 0.17 ± 0.02 | 0.94 |
| UAAC | 0.16 ± 0.05 | 0.15 ± 0.02 | 0.94 |
| UAAG | 0.13 ± 0.01 | 0.14 ± 0.01 | 1.1 |
| UAAU | 0.11 ± 0.01 | 0.12 ± 0.02 | 1.1 |
| UAGA | 0.09 ± 0.01 | 0.09 ± 0.01 | 0.96 |
| UAGC | 0.18 ± 0.02 | 0.18 ± 0.03 | 1.0 |
| UAGG | 0.13 ± 0.01 | 0.13 ± 0.02 | 0.96 |
| UAGU | 0.05 ± 0.01 | 0.06 ± 0 | 1.2 |
| UGAA | 0.21 ± 0.03 | 0.21 ± 0.01 | 1.0 |
| UGAC | 0.34 ± 0.04 | 0.53 ± 0.02 | 1.5 |
| UGAG | 0.13 ± 0.02 | 0.26 ± 0.02 | 2.0 |
| UGAU | 0.05 ± 0 | 0.06 ± 0.01 | 1.1 |
a
Percent readthrough is expressed as the mean ± standard deviation.
b
All measurements were carried out using the [psi−] strain 614 with or without pDB773, which expresses eRF1 from the SUP45 promoter.