Table 2.
Primers and PCR data
| Primer | Sequence (5’ – 3’) | Tm (°C) | Product size (bp) | GB reference/locus |
|---|---|---|---|---|
| X639_up | GTTAAAAACTGGGATATTCA | 55.0 | 1280 | XM_002996639 NCER_100183 |
| X639_low | ACAAATCTATCTCTTATCCCT | |||
| X580_up | AAGGGAAATATTAGGCAACTG | 56.6 | 1138 | XM_002996580 NCER_100253 |
| X580_low | CGGGAAGTTCAATTACACTC | |||
| X802_up | TGCCGAGTGATAAACTTACT | 58.0 | 873 | XM_002996802 NCER_100040 |
| X802_low | AATCGATATATCTGCGTTCTT | |||
| X754_up | ATGGCTTCTACAATTTACTTA | 55.0 | 1123 | XM_002996754 NCER_100059 |
| X754_low | AAAACATATTCGTGCACTA | |||
| X696_up | ATTTGAAATTGTCTCCCTATG | 55.0 | 1217 | XM_002996696 NCER_100117 |
| X696_low | GGAAATTACTTCGTCAACCT |
Primers used in PCR and sequencing, PCR annealing temperature (°C), product DNA size (bp) and GenBank reference sequences, together with the correspondent locus name