Table 2.

Summary of genes mutated in 2 or more case-matched tumour sites throughout disease course

Case ID#	Gene	PT	syn M[HEP] 1	syn M[HEP] 2	syn M[PUL] 1	syn M[PUL] 2	met M[HEP] 1	met M[HEP] 2	met M[PUL] 1	met M[PUL] 2	met M[PUL] 3
1	KRAS	G12D	G12D						G12D
	TP53	R342*	WT						R342*
2	APC	Q1338*	Q1338*		Q1338*	Q1338*
	KRAS	G12V	G12V		G12V	G12V
	SMAD4	R361C	R361C		R361C	R361C
3	APC	WT					R1450*		R1450*	WT	R1450*
		R876*					R876*		R876*	R876*	R876*
	KRAS	G12V					G12V		WT	G12V	G12V
	PIK3CA	WT					K111E		WT	WT	K111E
	TP53	R196*					R196*		R196*	R196*	R196*
4	TP53	R337C	R337C				R337C		R337C
		G199V	G199V				G199V		G199V
5	APC	P1319Lfs*	P1319Lfs*		P1319Lfs*	P1319Lfs*
	KRAS	G12A	G12A (9.7 % AF)		G12A	G12A
	PIK3CA	E542K	E542K		E542K	E542K
	TP53	G244C	G244C		G244C	G244C
6	APC	Q1367*					WT		Q1367*
	KRAS	G12V					WT		G12V
	SMAD4	Q250*					WT		Q250*
7	TP53	V157F	V157F				V157F	V157F	V157F
8	KRAS	G13D	G13D		G13D				G13D
	TP53	R248Q	R248Q		R248Q				R248Q
9	APC	R1114*	R1114*						R1114*	R1114*	R1114*
	TP53	R282W	R282W						R282W	R282W	R282W
10	APC	T1556Nfs*					T1556Nfs*		T1556Nfs*
	FGFR3	P718S					P718H		P718S
	TP53	V157G					V157G		V157G
11	TP53	R273C	R273C						R273C	R273C
12	APC	WT	L1488Tfs*						L1488Tfs*	L1488Tfs*
	KRAS	G12A	G12A						G12A	G12A
13	ABL1	WT	WT		P315S	P315S
	APC	R876*	WT		R876*	WT
	APC	E1284*	WT		E1284*	E1284*
	ERBB4	WT	WT		D300N	D300N
	KRAS	G12D	p.G12V		G12V (5.6 % AF)	G12V
14	APC	T1430Pfs*	T1430Pfs*	T1430Pfs*	T1430Pfs*
	NRAS	Q61R	Q61R	Q61R	Q61R
	TP53	R175H	R175H	R175H	R175H

AF allele frequency