3.

(A) Schematic diagram of the fabrication process of gold nanoshelled microcapsules (GNS-MCs). a: electrostatic adsorption of positive charged poly allylamine hydrochloride (PAH) onto microcapsules generated by double-emulsion method; b: deposition of GNPs onto PAH-coated microcapsules; c: formation of GNSs by the surface-seeding method; d: lyophilization to sublimate the encapsulated water in the inner aqueous phase of the microcapsules to produce small hollow spaces. (B) In vivo ultrasonograms in the rabbit right kidney showed US enhancement after administration of GNS-MCs. (C) Fluorescent microscopic images of HeLa cells with both agent and laser treatment stained by calcein AM indicated the photothermal cytotoxicity of GNS-MCs. (D) CPS contrast and B-mode images of the tumor after injection of GNS-MCs. (E) Quantitative measurement of tumor volumes after different treatments of each group demonstrated the excellent anti-tumor efficacy of GNS-MCs under the NIR light irradiation. Reproduced with permission from Ref. 63. Copyright 2013 Royal Society of Chemistry.