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. 2004 Sep;24(17):7392–7401. doi: 10.1128/MCB.24.17.7392-7401.2004

FIG. 3.

FIG. 3.

U6-p110 is required for splicing in vitro only under recycling conditions. MINX pre-mRNA was spliced in vitro at concentrations of 10 ng (1×; lanes 2 to 7), 50 ng (5×; lanes 8 to 13), and 100 ng (10×; lanes 14 to 19) per 25-μl reaction in mock-depleted nuclear extracts (lanes 2, 3, 8, 9, 14, and 15), p110-depleted nuclear extracts (lanes 4, 5, 10, 11, 16, and 17), and p110-depleted nuclear extracts complemented with 500 ng of recombinant p110 (lanes 6, 7, 12, 13, 18, and 19). Input MINX pre-mRNA is shown in lane 1. Assays were carried out for 45 and 90 min. The positions of pre-mRNA, spliced product mRNA, and the first-exon intermediate are indicated on the left.