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. 2004 Sep;24(17):7483–7490. doi: 10.1128/MCB.24.17.7483-7490.2004

FIG. 5.

FIG. 5.

SMG-1 interacts with SMG-2. (A) Proteins immunoprecipitated (IP) by anti-SMG-2 (αSMG-2) antibodies were electrophoresed and probed on Western blots with anti-SMG-1 (αSMG-1) antibodies. WT, wild type. (B) Western blots of strains used in Fig. 5A probed with anti-SMG-1. (C) Proteins immunoprecipitated by anti-SMG-1 antibodies were electrophoresed and probed on Western blots with anti-SMG-2 antibodies. (D) Proteins immunoprecipitated by anti-SMG-2 antibodies were electrophoresed and probed on Western blots with anti-SMG-1 antibodies. SMG-1(+) and SMG-1(D1966A) are expressed from transgenes in the strains of lanes 4 and 5, respectively. smg-1(r861), smg-2(r908), smg-3(r930), smg-4(r1169), and smg-5(r860) are null alleles of the respective genes.