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Western blot analysis of cell cycle proteins from mouse epidermis. Protein lysates of epidermis samples from K5-Myc, K5-Myc/CDK4−/−, K5-Myc/CDK4+/−, CDK4−/−, CDK4+/−, and wild-type siblings were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and blotted onto a nitrocellulose membrane. Primary antibodies against CDK4, CDK6, CDK2, cyclin D1, cyclin D2, cyclin A, and cyclin E were used for immunoblot analysis. Protein levels were quantified with a densitometer.
