Table 2.

Real-time-PCR primer sequences

Gene	Forward primer	Reverse primer
OPG	5′-GGGCGTTACCTGGAGATCG-3′	3′-CGTTGTCATGTGTTGCATTTCC-5′
RANKL	5′-CAGCATCGCTCTGTTCCTGTA-3′	3′-CTGCGTTTTCATGGAGTCTCA-5′
Ctnnb1	5′-CCTCCCAAGTCCTTTATGAATGG-3′	3′-CCGTCAATATCAGCTACTTGCTCTT-5′
PPARγ2	5′-GCCCTTTGGTGACTTTATGGA-3′	3′-GCAGCAGGTTGTCTTGGATG-5′
LPL	5′- GGGAGTTTGGCTCCAGAGTTT-3′	3′- CGTGTGTGAAATGTCATTGATCC-5′
Lep	5′- GTGGCTTTGGTCCTATCTGTC-3′	3′- CGTGTGTGAAATGTCATTGATCC-5′
AdipoQ	5′- TGTTCCTCTTAATCCTGCCCA-3′	3′- CCAACCTGCACAAGTTCCCTT-5
FASN	5′- AGAGATCCCGAGACGCTTCT-3′	3′-GCTTGGTCCTTTGAAGTCGAAGA-5′
Runx2	5′- CAGCATCCTATCAGTTCCCAA-3′	5′- CAGCGTCAACACCATCATT-3′
GAPDH	5′- GACTTCAACAGCAACTCCCAC-3′	3′- TCCACCACCCTGTTGCTGTA-5′

OPG osteoprotegrin, RANK-L Receptor activator of nuclear factor kappa-B ligand, Ctnnb1 beta-catenin, PPAR γ2 Peroxisome proliferator-activated receptor gamma-2, LPL lipoprotein lipase, Lep leptin, AdipoQ adiponectin, FASN fatty acid synthase, Runx2 Runt-related gene 2, GAPDH glyceraldehyde 3-phosphate dehydrogenase