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Effects of bile acid treatment on association of HATs, corepressors, and Swi/Snf with the endogenous CYP7A1 promoter. (A) Schematic diagram of the CYP7A1 gene. (B to F) ChIP assays were performed as described in Materials and Methods. Chromatin DNA precipitated by the antibodies was analyzed by PCR using primer sets specific for the promoter or control regions (indicated by arrows in panel A). Consistent results from >3 ChIP assays in each case were obtained. +, present; −, absent.
