FIG. 3.

Bile acid-induced chromatin remodeling is reversed by overexpression of Swi/Snf ATPase mutants. HepG2 cells were transfected with mammalian expression plasmids for ATPase mutants of Brm/Brg-1 or empty vector by electroporation, and 36 h later the transfected cells were treated with CDCA or DMSO for 12 h and further subjected to restriction enzyme accessibility assays as described in Materials and Methods. Briefly, DNA in intact nuclei was partially digested with either FokI or HincII (10 to 25 U/100 μl) by incubation at 37°C for 15 min, and genomic DNA was purified and then digested to completion with AvaII in vitro. One microgram of purified genomic DNA was subjected to LMPCR analysis using the primer set A as indicated in the schematic diagram at the bottom. +, present; −, absent.