FIG. 4.

Association of mSin3A-Swi/Snf-Brm with SHP in HepG2 cells. (A) HepG2 cell extracts were subjected to immunoprecipitation (IP) using IgG or antibodies against Brm. Association of mSin3A with the anti-Brm precipitate was detected by Western blotting using mSin3A antiserum. Conversely, the cell extracts were incubated with either IgG or mSin3A antiserum, and association of Brm with the anti-mSin3A precipitate was detected by antiserum against Brm. Input, 5% of total cell lysates. (B) HepG2 cells were transfected with Gal4DBD-SHP by electroporation and subjected to CoIP. The association of SHP with Brm was detected by Western blotting using Gal4DBD antibody. Conversely, the cell extracts were immunoprecipitated with either IgG or Gal4DBD antibody. Association of Brm and mSin3A with SHP was detected by Western blotting.