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. 2016 Oct 19;13:173. doi: 10.1186/s12985-016-0631-2

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Identification of SVV HB-CH-2016 strain. a The cytopathic effect of BHK-21 cells infected with SVV HB-CH-2016 strain at 18 h post-infection. b Plaque morphology in BHK-21 cells infected with fourth-passage SVV HB-CH-2016 strain at 48 h post-infection. c Immunofluorescence assay (IFA) of BHK-21 cells infected with SVV HB-CH-2016 strain at 12 h post-infection. Cells were stained with primary antibody using home-made mouse anti-SVV VP1 polyclonal antibodies. d Western blot analysis of BHK-21 cells infected with SVV HB-CH-2016 strain at 12 h post-infection. Cells were stained with primary antibody using a home-made mouse polyclonal anti-SVV VP1 antibody and mouse anti-tubulin antibody