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. 2016 Oct 18;9:219. doi: 10.1186/s13068-016-0638-3

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Design and screening of tetracycline-inducible promoter variants with catP as a reporter. Shown in a are the sequences of the original Tet3n0 promoter and seven synthetic inducible promoters. The randomized non-conserved bases in −35 and −10 promoter elements are highlighted in red. Shown in b is the activity of catP reporter under tet3no and inducible promoters 1–12. The catP activity is expressed in Units/g protein. The inset shows the catP activity of weak promoters on a smaller scale