Table 2.
List of oligonucleotides used in this study
| Oligonucleotide | Sequence (5′ → 3′) | Purpose |
|---|---|---|
| Og01 | GATTATAAGCGGCCGGCCATAAACTATTTTTTAAAGATAAAAGCT | PCR amplification of 5′-HA of adh |
| Og02 | TACGCCGCCAGGTTTAACCAAAACCAGCTTGGACTTCTAAAGA | PCR amplification of 5′-HA of adh |
| Og03 | TCTTTAGAAGTCCAAGCTGGTTTTGGTTAAACCTGGCGGCGTA | PCR amplification of 3′-HA of adh |
| Og04 | CAACTTGCCCACTGGCCGGCCTGACTATTTCACTATGAGTAAATGGT | PCR amplification of 3′-HA of adh |
| Og05 | GAATGTGTTTAAACTCTCTGAAACTAGCAAATTTGG | PCR amplification of 5′-HA of 2,3bdh |
| Og06 | GAGATAATTATGAAAGCTGTATTGTGGTTGTAAAAGAAGGATTTGAAACAC | PCR amplification of 5′-HA of 2,3bdh |
| Og07 | GTGTTTCAAATCCTTCTTTTACAACCACAATACAGCTTTCATAATTATCTC | PCR amplification of 3′-HA of 2,3bdh |
| Og08 | AAAGGAGTTTAAACGAAAGTGAGCTTTTTGGTTATGAAAA | PCR amplification of 3′-HA of 2,3bdh |
| Og09 | TATTAACCTTATAAAGTCCTACCCC | For screening |
| Og10 | TAATCCTCCTCTTATAGTTTTGTGA | For screening |
| Og11 | CAAAAGCTATACTTAGTGCTAGATT | For screening |
| Og12 | TCATTTCTCTATCTTCAAAAAGTGT | For screening |
| Og13 | AGCTGTAGATAACAATGGGATCAT | For screening |
| Og14 | GTGAGATATAATGAGAAACCTGATCC | For screening |
| Og15 | AATGGCAGGGCAGATAATTGTAATG | For screening |
| Og16 | AAGGCATTCTGAGCCAGTTCTTTTA | For screening |
| Og17 | TAACGTCCTTAACTATTTATCAATTCGATCGACTAT | To construct variants of ptet3no |
The table also includes the purpose of each oligonucleotide