Table 2.
Oligonucleotide | Sequence (5′ → 3′) | Purpose |
---|---|---|
Og01 | GATTATAAGCGGCCGGCCATAAACTATTTTTTAAAGATAAAAGCT | PCR amplification of 5′-HA of adh |
Og02 | TACGCCGCCAGGTTTAACCAAAACCAGCTTGGACTTCTAAAGA | PCR amplification of 5′-HA of adh |
Og03 | TCTTTAGAAGTCCAAGCTGGTTTTGGTTAAACCTGGCGGCGTA | PCR amplification of 3′-HA of adh |
Og04 | CAACTTGCCCACTGGCCGGCCTGACTATTTCACTATGAGTAAATGGT | PCR amplification of 3′-HA of adh |
Og05 | GAATGTGTTTAAACTCTCTGAAACTAGCAAATTTGG | PCR amplification of 5′-HA of 2,3bdh |
Og06 | GAGATAATTATGAAAGCTGTATTGTGGTTGTAAAAGAAGGATTTGAAACAC | PCR amplification of 5′-HA of 2,3bdh |
Og07 | GTGTTTCAAATCCTTCTTTTACAACCACAATACAGCTTTCATAATTATCTC | PCR amplification of 3′-HA of 2,3bdh |
Og08 | AAAGGAGTTTAAACGAAAGTGAGCTTTTTGGTTATGAAAA | PCR amplification of 3′-HA of 2,3bdh |
Og09 | TATTAACCTTATAAAGTCCTACCCC | For screening |
Og10 | TAATCCTCCTCTTATAGTTTTGTGA | For screening |
Og11 | CAAAAGCTATACTTAGTGCTAGATT | For screening |
Og12 | TCATTTCTCTATCTTCAAAAAGTGT | For screening |
Og13 | AGCTGTAGATAACAATGGGATCAT | For screening |
Og14 | GTGAGATATAATGAGAAACCTGATCC | For screening |
Og15 | AATGGCAGGGCAGATAATTGTAATG | For screening |
Og16 | AAGGCATTCTGAGCCAGTTCTTTTA | For screening |
Og17 | TAACGTCCTTAACTATTTATCAATTCGATCGACTAT | To construct variants of ptet3no |
The table also includes the purpose of each oligonucleotide