FIG. 2.
Thermosensitive mutants of spb70+. (A) Thermosensitivity of spb70 mutants. Wild type (wt), cdc45goa1, spb70-U01, spb70-U02, spb70-U03, and spb70-U04 were first cultured in liquid YES medium at 25°C. Equal numbers of log-phase cells were spotted on YES plates in threefold serial dilution and incubated at 25, 30, and 36°C for 3 days. (B) FACS profile of spb70-U02. cdc10-M17, spb70-U02, and cdc17-K42 were first grown at 25°C in YES medium followed by shifting to 36°C for 4 h. cdc10 and cdc17 were used as 1C and 2C DNA content standards, respectively. (C) Terminal phenotype of spb70-U02. Exponentially growing cells were shifted to 36°C for 4 h and stained with DAPI.