Skip to main content
. 2004 Sep;24(17):7419–7434. doi: 10.1128/MCB.24.17.7419-7434.2004

FIG. 6.

FIG. 6.

Phosphorylation and unphosphorylation of Orp2 affects the temperature-sensitive growth of spb70 mutants. (A) Mutants orp2-T4A and orp2-T3D exert opposite effects on spb70-U05. Single mutants orp2-T4A, orp2-T3D, and spb70-U05 and double mutants spb70-U05 orp2-T4A and spb70-U05 orp2-T3D were cultured in liquid YES medium at 25°C. Equal numbers of log-phase cells from each strain were spotted on YES plates in threefold serial dilution and incubated at 25 and 35°C for 2 days. (B) orp2-T4A, but not orp2-T3D, suppresses the temperature sensitivity of spb70-U05 at 36.5°C. Thermosensitivity of double mutant spb70-U05 orp2-T4A was compared to single mutant spb70-U05 and double mutant spb70-U05 orp2-T3D after 3 days of incubation at 36.5°C. (C) Suppression of the temperature sensitivity of spb70-U12 by orp2-T4A. Single mutants orp2-T4A and spb70-U12 and double mutant spb70-U12 orp2-T4A were incubated at 30°C for 2 days.