TABLE 2.
spb70 mutants
| Mutant | Allelea | Growth at (°C)b:
|
|
|---|---|---|---|
| 25 | 30 | ||
| spb70-U01 | Q469R | ++ | + |
| spb70-U02 | L369S, H471Y | ++ | − |
| spb70-U03 | F450L, N527H | ++ | +/− |
| spb70-U04 | Q469K | ++ | + |
| spb70-U05 | H349L, V523A | ++ | ++ |
| spb70-U12 | L442P, N485S | ++ | + |
| spb70-U13 | S314P, 3 others | ++ | ND |
| spb70-U14 | G311R, S314P | +/− | − |
| spb70-U15 | S314P, 3 others | ++ | ND |
a
Bold allele is the mutation responsible for temperature sensitivity; italic allele causes constitutive growth defect.
b
++, no growth defect; +, mild growth defect; +/−, significant growth defect; −, no growth; ND, not determined. No growth was observed with any mutant at 36°C.