TABLE 2 .

Effect of sulfur limitation on gene expression in stationary phase^a

Locus tag	Gene	Description of product	Fold change (S-limited/control) in expression ofb:
			mRNA	Protein
SAR11_0007	hflC	Integral membrane proteinase	0.20	1.34
SAR11_0162	groEL	Chaperonin	0.18
SAR11_0173		2-Hydroxy-6-oxo-2,4-heptadienoate hydrolase	0.64	1/∞
SAR11_0287	ccmC	Heme exporter membrane protein	4.74	ND
SAR11_0399	Rbr	Rubrerythrin; peroxidase	0.17	2.24
SAR11_0756	aldA	Acetaldehyde dehydrogenase	0.23	0.63
SAR11_0864		Hypothetical protein	0.22	0.62
SAR11_0865		Mannitol/chloroaromatic compound transport	0.19
SAR11_1163		Hypothetical protein	4.69	ND
SAR11_1164		Hypothetical protein	7.67	ND
SAR11_1172c	osmC	Organic hydroperoxidase	0.23	0.21
SAR11_1274	cspL	Cold shock DNA-binding protein	0.18	1.27
SAR11_1302	opuAC	Glycine betaine ABC transporter: periplasmic	0.22	0.62
SAR11_1305	glnT	Glutamine synthetase	0.19	0.78
SAR11_1361	livJ2	Leu/Ile/Val-binding transport system	0.23

^aAll 15 genes with fourfold or greater differences in mRNA or protein expression between sulfur-limited stationary-phase (n = 5) and control stationary-phase (n = 4) cultures are listed.

^bValues greater than 1 indicate higher abundance in sulfur-limited condition. Differences in expression unsupported by a P value of 0.05 or less are omitted. Boldface indicates values that were significantly different (P ≤ 0.05) after correcting for multiple comparisons. ND, not detected by mass spectrometry at any time point, potentially due to methodological limitations on extracting insoluble proteins, such as those localized to the membrane; 1/∞, observed in sulfur-limited stationary samples but not detected in control stationary samples.

^cDownstream from a SAM-V riboswitch.