TABLE 1.
Plasmids used in this study
| Plasmid | Description | Mutations or comments | Source or reference |
|---|---|---|---|
| pSD120 | TLC1 CEN URA3 | D. Gottschling | |
| pA258 | tlc1-Δ148-440 CEN URA3 | Δ148-440 | 33 |
| pVL2527 | tlc1-Δ148-440 URA3 | tlc1-Δ148-440 subcloned into YIp211 | pA258, YIp211 |
| pVL369 | ADH-EST2 2μm URA3 | 32a | |
| pVL795 | TLC1 CEN TRP1 | 2.6-kb TLC1 gene in pRS414 | This work |
| pVL1221 | CDC13-EST1 CEN HIS3 | In-frame fusion between CDC13 and EST1 in pRS414 | 15 |
| pVL1881 | TLC1S. castellii 2μm TRP1 | 7.2-kb genomic insert from S. castellii in YEp112 | This work |
| pVL1882 | TLC1S. bayanus 2μm TRP1 | 2.6-kb genomic insert from S. bayanus in YEp112 | This work |
| pVL1883 | TLC1S. kluyveri 2μm TRP1 | 6.9-kb genomic insert from S. kluyveri in YEp112 | This work |
| pVL2090 | tlc1-29 CEN TRP1 | TAGA753-756 → ATCT | pVL795 |
| pVL2091 | tlc1-30 CEN TRP1 | TCTA807-810 → AGAT | pVL795 |
| pVL2099 | tlc1-31 CEN TRP1 | TAGA753-756 → ATCT + TCTA807-810 → AGAT | pVL795 |
| pVL2122 | tlc1-34 CEN TRP1 | TAGATTTTT753-761 → ATCTAAAAA | pVL795 |
| pVL2175 | tlc1-43 CEN TRP1 | GATTT755-759 → CTAAA | pVL795 |
| pVL2126 | tlc1-44 CEN TRP1 | AAATC804-808 → TTTAG | pVL795 |
| pVL2176 | tlc1-45 CEN TRP1 | GATTT755-759 → CTAAA + AAATC804-808 → TTTAG | pVL795 |
| pVL2128 | tlc1-47 CEN TRP1 | Δ660-664 | pVL795 |
| pVL2192 | tlc1-59 CEN TRP1 | Δ751-812 | pVL795 |
| pVL2195 | tlc1-62 CEN TRP1 | Δ762-795 | pVL795 |
| pVL2428 | tlc1-64 CEN TRP1 | Δ728-739 | pVL795 |
| pVL2208 | tlc1-65 CEN TRP1 | CCGT741-744 → GGTA | pVL795 |
| pVL2209 | tlc1-66 CEN TRP1 | ATGG721-725 → TGCC | pVL795 |
| pVL2210 | tlc1-67 CEN TRP1 | CCGT741-744 → GGTA + ATGG721-725 → TGCC | pVL795 |
| pVL2218 | tlc1-71 CEN TRP1 | GTTT743-746 → TAAA | pVL795 |
| pVL2214 | tlc1-72 CEN TRP1 | AAAT719-722 → TTTG | pVL795 |
| pVL2215 | tlc1-73 CEN TRP1 | GTTT743-746 → TAAA + AAAT719-722 → TTTG | pVL795 |
| pVL2216 | tlc1-74 CEN TRP1 | Δ813-822 | pVL795 |
| pVL2204 | tlc1-76 CEN TRP1 | Replacement of nt 751-812 with analogous sequences from TLC1S. kluyveri | pVL795 |
| pVL2245 | tlc1-91 CEN TRP1 | Replacement of nt 717-812 with analogous sequences from TLC1S. kluyveri | pVL795 |
| pVL2249 | tlc1-94 CEN TRP1 | Replacement of nt 717-812 with nt 59-98 of O. nova telomerase RNA | pVL795 |
| pVL2251 | tlc1-96 CEN TRP1 | Replacement of nt 717-812 with nt 90-183 of hTR | pVL795 |
| pVL2430 | tlc1-111 CEN TRP1 | Δ717-724 | pVL795 |
| pVL2339 | tlc1-112 CEN TRP1 | Δ717-748 | pVL795 |
| pVL2426 | tlc1-120 CEN TRP1 | Δ671-703 | pVL795 |
| pVL2611 | tlc1-122 CEN TRP1 | Δ717-812 | pVL795 |
| pVL2431 | tlc1-124 CEN TRP1 | Δ823-828 | pVL795 |
| pVL2432 | tlc1-125 CEN TRP1 | Δ829-833 | pVL795 |
| pVL2435 | tlc1-126 CEN TRP1 | Δ710-716 | pVL795 |
| pVL2434 | tlc1-127 CEN TRP1 | Δ704-709 | pVL795 |
| pVL2497 | tlc1-129 CEN TRP1 | Derived from pVL2245 with AGGTGGCTACAGTATTCGACG deleted | pVL2245 |