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. 2004 Sep;24(17):7359–7369. doi: 10.1128/MCB.24.17.7359-7369.2004

FIG. 3.

FIG. 3.

Overexpression of TLR4 and MD2 activates SPK. (A) The relative SPK activity in HEK 293 cells (HEK), HEK 293 stably transfected with TLR4 (HEK-TLR4), and HEK 293 stably transfected with both TLR4 and MD2 (HEK-TLR4/MD2). Cells were serum starved overnight and SPK activity in cell lysate was assayed. (For HEK 293 cells, relative SPK activity of 100% = 36 pmol/min/mg of protein). (B) Relative sphingosine and S1P levels in HEK 293 cells (HEK) and HEK 293 cells stably transfected with TLR4 and MD2 (HEK-TLR4/MD2). Cells were serum starved overnight, and relative levels of total cellular sphingosine (Sph) and S1P were determined.