FIG. 3.

Effects of carbon source on lacZ expression mediated by UAS1, UAS2, and their subregions. UAS1 is subdivided into UAS1-1 and UAS1-2, whereas UAS2 is subdivided into UAS2-1 and UAS2-2. Nrg1 and Sfl1 act on UAS1-1 and UAS2-2, respectively, and the transcriptional activators required for activation of UAS1-2 and UAS2-1 are indicated. The pLG-UAS series was transformed into wild-type cells, and the resulting transformants were grown to mid-log phase in synthetic medium containing 2% glucose (R) or 2% glycerol-ethanol (D). β-Galactosidase activity was determined on three independent colonies.