FIG. 6.

The Srb8-11 complex is required for NRG1 and SFL1 expression. (A) Plasmids expressing Nrg1-HA or Sfl1-HA, from their own promoters or from the ADH1 promoter, were transformed into wild-type (WT) and mutant cells. Transformants were grown to mid-log phase in synthetic medium containing 2% glucose. Protein extracts were prepared from each transformant, and 50 μg (own promoter) or 20 μg (ADH1 promoter) of extract was separated by SDS-PAGE for immunoblotting with an α-HA antibody. The same membranes were probed with an α-actin monoclonal antibody. (B) Plasmids containing NRG1p-lacZ and SFL1p-lacZ were transformed into wild-type and mutant cells. The cells were grown in synthetic medium containing 2% glucose, and β-galactosidase activity was measured with three independent colonies.