FIG. 5.

Genetic cyclin D1 deficiency increases PPARγ abundance in Apc^Min intestinal epithelium. (A) Immunohistochemical (IHC) staining of duodenal epithelia of Apc^Min mice. (B) Representative Western blot analysis of PPARγ1 in colonic epithelium in Apc^Min mice (comparisons were made with 10 animals [4 cyclin D1^+/+, 4 cyclin D1^+/−, and 2 cyclin D1^−/−] for each cyclin D1 genotype, two representative examples are shown for each genotype, and each lane represents lysate preparations made from individual animals with GDI as a loading control). (C) Analysis of mean PPARγ1 protein levels by densitometry (wt set to 1; mean and standard error of the mean; 13 weeks), normalized to GDI loading control. Asterisks indicate P values <0.05.