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. 2016 Oct;55(4):476–486. doi: 10.1165/rcmb.2015-0328OC

Figure 2.

Figure 2.

Effects of vinculin knockdown on thrombin– and IL-6–induced FA rearrangement, actin cytoskeletal remodeling, and endothelial permeability. Human pulmonary ECs were transfected with vinculin-specific (si-VNC) or nonspecific small interfering RNA. (A and B) FA (A) and cytoskeletal (B) remodeling in thrombin– (0.2 U/ml, 10 min) or IL-6/SR (30 ng/ml and 60 ng/ml, 5 h)–treated cells was analyzed by immunofluorescence staining for talin or F-actin, respectively. Paracellular gaps are marked by arrows. (C) Quantitative analysis of paracellular gap formation in control and treated HPAECs. Data are expressed as mean ± SD; n = 4; *P < 0.05 versus nonspecific RNA (ns-RNA). (D) HPAECs plated on microelectrodes were treated with thrombin or IL-6/SR followed by measurements of transendothelial electrical resistance (TER). Arrows in panel D indicate time point of agonist.