Figure 4.

Preexisting lung-restricted antibodies induce alloimmunity after murine lung transplantation. Wild-type donor BALB/c lungs were transplanted into C57BL/6 recipients. A combination of MHC-related 1 and cytotoxic T-lymphocyte–associated protein 4-Ig was used to induce tolerance. We administered Col-V (200 μg), KAT (200 μg), Col-V plus KAT (200 μg each), or isotype control antibodies on Days −2, −1, 0, 6, 7, and 8, and weekly thereafter until Post-transplant Days 30 and 45. Subsequently, development of allospecific antibodies was analyzed using flow cytometry by testing the recipient murine serum against (A) donor H-2k^d or (B) third-party H-2k^k splenocytes on Days 30 and 45. The increase in anti-H-2k^d compared with anti-H-2k^k was statistically significant (P < 0.01). Data are presented as mean (±SEM). Abs, antibodies.