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. 2016 Oct 19;47:101. doi: 10.1186/s13567-016-0386-1

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Acid secretion by parietal cells. LysoSensor™ Yellow/Blue DND-160 was used to monitor the acid secretion by live parietal cells incubated either by Cim or His/IBMX. Cell Tracker Red CMTPX was used to track all live cells. Some apical vacuoles of parietal cells in resting stage revealed several small areas with weak yellow fluorescence, indicating basal acid secretion (A). Parietal cell treated with His/IBMX showed an expansion of apical vacuoles, as shown by strongly enlarged zones with yellow fluorescence, indicating the continuous secretion of acid (B). Cim: Cimitidine; His: histamine; IBMX: 3-isobutyl-1-methylxanthine.