Fig. 6.

ASIC blocker diminazen suppresses inward GABAergic currents. In the same series of experiments during each sweep evoked PSCs (2 PSCSs with 100 ms interval) were recorded below their reversal potential as inward currents (a), and above the reversal potential, as outward ones (b), see Methods for details. Superimposed traces of original current traces (averages of 10 sequential PSCs) before (solid lines) and after (dotted lines) diminazene (20 μM) application are shown on the right panel, summary graphs (n = 6) are shown on the left panel. PSC-amplitudes and paired-pulse ratios for inward PSCs (PSC2/PSC1) were normalized to control values (average for 20 PSCs preceding drug application). Normalized paired-pulse ratio is plotted in (c). The experiments were done in ‘HEPES 2’ solution. In control, absolute amplitudes of inward and outward currents (mean ± S.D) in this series of experiments were: -188.5 ± 97.0 pA; 178.4 ± 58.4 pA