Additional file 7. ADAPT workflow for automated analysis of cell migration. Shown is an example of the ADAPT plugin and associated trajectory visualization of the MCF-7 MT1-MMP C2 video (Additional file 5). Additional file 5 is shown as the overlay of zsGreen cells (green) and Alexa594 gelatin coating (red), as well as the individual channels (top). The zsGreen channel was used for the ADAPT analysis (bottom, left) to yield a trajectory visualization of individual cells from initial point of tracking (bottom, right). Three videos from independent experiments were analyzed in this manner and the trajectory visualization from each was used to quantify individual cell migration after 20 h. Cell migration data is compiled in Fig. 6b. Scale bars = 100 μm. (MOV 5300 kb)
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