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Additional file 9. Time-lapse analysis of 3D culture dynamics of MCF-7 MT1-MMP cells. Parental MCF-7 (Additional file 9), and MT1-MMP C1, C2, and C3 (Additional files 10, 11 and 12, respectively) cells were embedded in 50 % matrigel and incubated in a live imaging chamber at 37 °C, 5 % CO₂. Z-stacks (100 μm, 5 μm slices) were acquired using a Leica DM16000 B microscope. Frames were taken every 30 min for 72 h and focal planes showing colony features with the greatest clarity were compiled into time-lapse movies using ImageJ. Representative stills of each cell line at three different time points are shown in Additional file 8: Figure S3. Scale bars = 100 μm. (AVI 14794 kb)