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Additional file 14. Real-time intravital imaging of extravasated MCF-7 MT1-MMP C3 cells. MCF-7 MT1-MMP C3 cells stably expressing zsGreen (green) were injected intravenously into the CAM vasculature of day 13 chicken embryos and imaged 24 h post-injection after labeling the vessels with lectin rhodamine (red). A movie in real-time was acquired using the resonant scanner of a Nikon A1R+ confocal microscope. The focus of the microscope was moved manually to show that a single C3 cells has extravasated from the CAM vasculature and shows a protrusion into the stroma (devoid of lectin-rhodamine signal). Scale bar = 100 μm. (MOV 9808 kb)