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Additional file 4. Time-lapse analysis of ECM degradation and cell migration of MCF-7 MT1-MMP cells. Parental MCF-7 (Additional file 3), and MT1-MMP C1, C2, and C3 (Additional files 4, 5 and 6, respectively) cells stably expressing zsGreen (green) were seeded on Alexa594 gelatin coated coverslips (red) and incubated in a live imaging chamber at 37 °C, 5 % CO₂. Images were acquired using a Leica DM16000 B fluorescent microscope. Frames were taken every 10 min for 20 h and compiled into time-lapse movies using ImageJ. Representative stills of each cell line at time 0 and 20 h are shown in Fig. 6. Scale bars = 100 μm. (AVI 17197 kb)