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. 2016 Oct 18;35:163. doi: 10.1186/s13046-016-0442-8

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Micrograph representative of adherent cells and spheroid cells generated from SN12C cells by SFM. a The adherent cells. Bar = 25 μm. b Examples of spheroid cells isolated from SN12C adherent cells which were placed in the serum-free culture. Bar = 50 μm. c Spheres passaged continuously for three times. Representative staining of three independent experiments is shown