Fig. 2.

Tumorigenecity of spheroid and adherent cells in vivo. a1 Representative xenograft tumors derived from 10⁶,10⁷ adherent cells and 10⁴,10⁵,10⁶ spheroid cells. a2 BALB/C mice injected with different number of spheroid cells and adherent cells that developed tumors and the tumor volumes were quantified. b Representative xenograft tumors derived from 10⁵ cells. All mice formed tumors with spheroid cells, but not with adherent cells. c The H&E staining, immunostained with rat anti-mouse and mouse anti-human CD34 antibody of the tumors formed by spheroid cells and adherent cells (magnification × 100). Representative the blood microvessel density(MVD) and vasculogenic mimicry (VM) in tumors derived from spheroid cells and adherent cells was measured. The experiment has been repeated once with a similar outcome. (Magnification × 100). Representative staining of three independent experiments was shown