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. 2016 Oct 18;35:163. doi: 10.1186/s13046-016-0442-8

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Functional analysis of spheroid-derived endothelial cells (spheroid-EC). a Morphological analysis of spheroid cells, spheroid-EC differentiated at 10 ng/ml VEGF, and HUVEC. b Flow cytometry analysis of CD31 and CD34 expression in spheroid cells, spheroid-EC, and HUVEC. c1,c2 The comparative expression of CD31 and CD34 in spheroid cells, spheroid-EC, and HUVEC. d Assessment of network formation by spheroid cells, spheroid-EC, and HUVEC on Matrigel. e Fluorescence imaging of acetylated low-density lipoprotein (red) and spheroid cells, spheroid-EC, and HUVEC metabolizing ac-LDL